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alsT [2018-10-27 17:34:46]
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alsT [2018-10-27 17:34:46]

putative glutamine transporter
locus
BSU18120
pI
9.39
mw
50.14 kDa
protein length
465 aa Sequence Blast
gene length
1395 bp Sequence Blast
function
uptake of glutamine
product
putative glutamine transporter
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.3|Alanine or glycine cation symporter family]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,938,925 → 1,940,322

    The protein

    Protein family

  • sodium:alanine (SAF) symporter family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|GlnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|YrbD], [protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|YflA]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), membrane [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR]: repression, [pubmed|28835263], in [regulon|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR regulon]

    • regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]) [Pubmed|12823818]
  • repressed in the presence of glutamine ([protein|641C4BDD9702804642E1753A9C779E80FABB3919|GlnR]) [Pubmed|28835263]
    • view in new tab

    Biological materials

    Mutant

  • GP1888 (tet) available in [SW|Jörg Stülke]'s lab
  • BKE18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA
  • BKK18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC, downstream forward: _UP4_TAATATAAATATAAAACAAA

    • References

  • 12823818,25755103,18763711